Assessment of Genetic Fidelity of Micropropagated Gerbera (Gerbera jamesonii Bolus) Plants by ISSR Markers
نویسندگان
چکیده
منابع مشابه
Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures
A high frequency shoot organogenesis and plant establishment protocol has been developed for Gerbera jamesonii from ex vitro leaf derived callus. The optimal callus was developed on Murashige and Skoog (MS) basal medium supplemented with 0.4 mg L 6-benzylaminopurine (BAP), 4.0 mg L -naphthalene acetic acid (NAA) and 3% (w/v) sucrose. Two callus types differing in their structures and growth rat...
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Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accessio...
متن کاملAssessment of the genetic stability of micropropagated plants of Cannabis sativa by ISSR markers.
Inter-simple sequence repeat (ISSR) markers were used to evaluate the genetic stability of the micropropagated plants of Cannabis sativa over 30 passages in culture and hardening in soil for 8 months. A total of 15 ISSR primers resulted in 115 distinct and reproducible bands. All the ISSR profiles from micropropagated plants were monomorphic and comparable to mother plants, confirming the genet...
متن کاملRapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants
Rapid in vitro multiplication of shoots has been described using capitular sections and leaf explants in Gerbera jamesonii. Three types of basal media regimes were used including MS and two of its modified forms⎯MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular s...
متن کاملTetranychus urticae (Acari: Tetranychidae) on Gerbera jamesonii Bolus and Hook (Asteraceae).
Gerbera (Gerbera jamesonii Bolus and Hook,) is an ornamental Asteraceae of great commercial value, and pests can affect adversely its cultivation. More than 20 species of arthropods cause economic damage on gerbera, among them the two spotted mite, Tetranychus urticae Koch, 1836 (Acari: Tetranychidae), considered a key pest for this and other ornamental plants. In this work, some life-cycle asp...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2018
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2018.712.063